Résumé: Erythroid differentiation is an essential and continuous process leading to the production of red blood cells, that has long been used as a model to study the genetic basis of normal differentiation and disease. It relies on highly dynamic gene expression programs that are tightly linked to three-dimensional (3D) genome organization. This 3D organization, formed as series of ‘chromatin loops’ and topological domains (TADs), allows the establishment of proper long-range contacts between genes and their distal regulatory elements, and prevents ectopic contacts between elements located in separate domains. Importantly, this organization was shown to be disrupted in several diseases, including cancer and leukemia. We are using erythropoiesis as a model to study the molecular mechanisms controlling chromatin looping, and the dynamics of 3D genome organization in vivo. To this aim we use mouse and human erythropoiesis to undertake a multidisciplinary approach combining high throughput molecular genomic approaches (ChIP-Seq, 4C-Seq and Hi-C) and proteomics analysis of transcription and chromatin regulatory factors.
Selected recent publications - Eric SOLER, Ph.D.
Dao LTM, Galindo-Albarrán AO, Castro-Mondragon JA, Andrieu-Soler C, Medina-Rivera A, Souaid C, Charbonnier G, Griffon A, Vanhille L, Stephen T, Alomairi J, Martin D, Torres M, Fernandez N, Soler E, van Helden J, Puthier D, Spicuglia S.
Nat Genet. 2017 Jul;49(7):1073-1081.
Brouwer RW, van den Hout MC, van IJcken WF, Soler E, Stadhouders R.
Methods Mol Biol. 2017;1507:199-220.
Stadhouders R, Cico A, Stephen T, Thongjuea S, Kolovos P, Baymaz HI, Yu X, Demmers J, Bezstarosti K, Maas A, Barroca V, Kockx C, Ozgur Z, van Ijcken W, Arcangeli ML, Andrieu-Soler C, Lenhard B, Grosveld F, Soler E.
Nat Commun. 2015 Nov 23;6:8893.
van den Heuvel A, Stadhouders R, Andrieu-Soler C, Grosveld F, Soler E.
Blood. 2015 Mar 5;125(10):1521-5. doi: 10.1182/blood-2014-11-567925.
Stadhouders R, Aktuna S, Thongjuea S, Aghajanirefah A, Pourfarzad F, van Ijcken W, Lenhard B, Rooks H, Best S, Menzel S, Grosveld F, Thein SL, Soler E.
J Clin Invest. 2014 Apr;124(4):1699-710. doi: 10.1172/JCI71520.
Stadhouders R, Kolovos P, Brouwer R, Zuin J, van den Heuvel A, Kockx C, Palstra RJ, Wendt KS, Grosveld F, van Ijcken W, Soler E.
Nat Protoc. 2013 Mar;8(3):509-24. doi: 10.1038/nprot.2013.018.